There is a part 2 video where he addresses this: theoretically, yes, practically, you would have to compose 99 original (original/unique enough for ContentID to

There is a part 2 video where he addresses this: theoretically, yes, practically, you would have to compose 99 original (original/unique enough for ContentID to match them) tracks and include a 20 seconds snippet of each for it to work. He stuck with a single one, because he started using it for his outro. Theoretically he could compose another for the intro and make it 66:33, but after that, I have a feeling the viewers would start dropping off rather quickly.