As for methods here's the list: https://animal.research.uiowa.edu/iacuc-guidelines-euthanasia https://animal.research.uiowa.edu/iacuc-guidelines-euthanasi... I

As for methods here's the list: https://animal.research.uiowa.edu/iacuc-guidelines-euthanasia https://animal.research.uiowa.edu/iacuc-guidelines-euthanasi... In my work, our approved methods were isoflurane and decapitation. In some types of experiments CO2 will interfere with your result.