Definitely depends on the audience. If you know your site/app will be used on older machines or by less tech savvy folks, it's often safer to stick with 800x60

Definitely depends on the audience. If you know your site/app will be used on older machines or by less tech savvy folks, it's often safer to stick with 800x600 in those cases. Otherwise, do some assessment and decide based on that. If there's no reason to exclude people though, then you may as well include as many as you can. Older browsers (IE6 anyone?) can be cut on newer things as well if the time to test and ensure it works in them isn't worth it, but that depends on your audience again. Then there's the accessibility question. You're not developing for users within public or govt institutions are you? ;)