3 ms·
"each run is tens of thousands of dollars" I'm not familiar with sequencing economics, would you mind explaining the cost of a "run"?
by dpflan 7y ago
"each run is tens of thousands of dollars"
I'm not familiar with sequencing economics, would you mind explaining the cost of a "run"?
- lkirk 7y agoA sequencing run (at least in the context of Illumina's technology) requires a few very expensive consumable reagents: First being the flowcell (microscope slide that the dna sticks to while being read by a laser), the reagents (containing enzymes with fluorophores and other reagents for amplifying and manipulating DNA), and the actual power consumed by such machinery. This does not factor in prep/lab costs (which can be kept at a minimum with automation, but that also is a high startup cost endeavor). Each sequencing run can take ~1-3 days depending on the format. Edit: this video may be able to explain a little better how this process works: https://www.youtube.com/watch?v=fCd6B5HRaZ8 https://www.youtube.com/watch?v=fCd6B5HRaZ8
- dpflan 7y agoThanks for the explanation. I’ve seen research on graphene-based nanopore sequencing, but my knowledge and understanding are shallow.