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How is CRISPR used for genome synthesis? I thought it was all driven by synthesis, and overlapping homology of sticky ended fragments, and ligase, but maybe tha
by inciampati 7y ago
How is CRISPR used for genome synthesis? I thought it was all driven by synthesis, and overlapping homology of sticky ended fragments, and ligase, but maybe that's the old days.
- folli 7y agoIt's not used for synthesis. It's very helpful for modifications of existing genomes, but it has no relevance in commercial DNA synthesis (which is a purely chemical process).