4 ms·
> samples were peripheral blood mononuclear cells Yeah, I was expecting to see flow cytometric cell sorting (1) for monocytes on CD14 and CD64 (2), which I wou
by killjoywashere 8y ago
> samples were peripheral blood mononuclear cells
Yeah, I was expecting to see flow cytometric cell sorting (1) for monocytes on CD14 and CD64 (2), which I would believe to be monocytes.
The "sample preparation" procedure they describe (3) is ... somewhat lacking in specificity. They pelletized the sample three times. I'm not even sure those cells are intact, and, mechanistically, the sample no doubts includes granulocytes, lymphocytes, and monos, so who knows what's going on there.
(1) https://www.labome.com/method/Flow-Cytometry-and-Cell-Sorting-A-Practical-Guide.html https://www.labome.com/method/Flow-Cytometry-and-Cell-Sortin...
(2) https://www.ncbi.nlm.nih.gov/pubmed/9844593 https://www.ncbi.nlm.nih.gov/pubmed/9844593
(3) https://www.pnas.org/content/pnas/suppl/2019/04/25/1901274116.DCSupplemental/pnas.1901274116.sapp.pdf https://www.pnas.org/content/pnas/suppl/2019/04/25/190127411...