* Extract RNA * Reverse-transcribe this into DNA * PCR amplify target sequence using custom primers. Generally you design the primers to create restriction s

* Extract RNA * Reverse-transcribe this into DNA * PCR amplify target sequence using custom primers. Generally you design the primers to create restriction sites flaking the sequence of interest. * Restriction digest the amplicons * Pop that into a cloning vector, e.g. Gateway * Clone and isolate a colony * Sanger sequence to confirm you've got what you want * Move construct into expression vector * Transform yeast with expression vector construct * Sequence some colonies to find one that worked well (these vectors use resistance genes to provide an easy screening process) * Once you've confirmed it worked, you're all set. You can just use plasmids or do a stable transformation; the latter is harder but it's what you'd want for commercial production I think.