3 ms·
Step 1 is you hope that someone already identified the gene(s) that do what you want. You look it up in a database and copy-paste the DNA sequence into a word d
by jakobegger 8y ago
Step 1 is you hope that someone already identified the gene(s) that do what you want. You look it up in a database and copy-paste the DNA sequence into a word document.
Step 2 you adjust the DNA sequence for the target organism. This might mean using different codons, changing the start of the gene, etc. Usually you also append a few extra genes like GFP or antibiotic resistance genes that make working with your DNA sequence easier.
Step 3 upload your DNA sequence to an online store for DNA synthesis. A few days later you'll get an envelope with a small plastic vial with your synthesized DNA.
Step 4 Now you need to get your DNA into the target organism. I'm not sure how you do that for yeast, but there's probably a well known protocol you can follow. You might also first put your DNA into bacteria to make more of it.
Step 5 now grow your cells, and put them under the microscope, check if they glow green (that's why you put the GFP in there) and perform various other analysis to see if what you did works. It probably didn't, so you go back and change something, and try again.