> "Unfortunately, one of the rats accidentally drowned during swimming training; therefore, the final animal number of the [experimental group] was 17." Damn,

> "Unfortunately, one of the rats accidentally drowned during swimming training; therefore, the final animal number of the [experimental group] was 17." Damn, poor guy. > "When training, a screw nut approximately 3% of their mean body weight was tied to the top end of the tail of each rat, and all rats were kept in the special pools with water of 60 cm depth (water temperature 25–30°C, pH 6.5–7.0) until fatigued (submerged below the surface for five seconds twice). The fatigued rats ceased training immediately, were removed from the water for a short break, showered with running water and then dried with hair dryers." So, systematically drowning rats until they aspirate water, every day for twelve weeks. And they showed negative respiratory effects. Huh.