Great question. There's some debate, apparentl, but the basic result is that temperature variation has side-effects, but superfreezing (keeping temp below < -2

Great question. There's some debate, apparentl, but the basic result is that temperature variation has side-effects, but superfreezing (keeping temp below < -20F, or below 0F, depending on the studied and what is studied) avoids the problems. https://pubs.acs.org/doi/abs/10.1021/ie50464a017 https://pubs.acs.org/doi/abs/10.1021/ie50464a017