8 ms·
This is very cool. I hope you get some cool uses out of this. What's the max frame rate you can get out of the cameras (without tiling)? Are you able to contro
by ak217 8y ago
This is very cool. I hope you get some cool uses out of this.
What's the max frame rate you can get out of the cameras (without tiling)? Are you able to control focus and camera gain?
- kasbah 8y agoThanks, working out the max frame rate is a bit complicated by the fact it uses time delay integration (TDI) scanning. There are 4 TDI cameras each 2048 * 128 pixels. I couldn't find any official documentation (the cameras are Hamamatsu S10405 CCDs and there is one C10000-509 control board for 2 cameras each). I think it's the same sensor as this camera which quotes the line rate at 50khz. https://www.hamamatsu.com/resources/pdf/sys/SCAS0085E_C10000-801.pdf https://www.hamamatsu.com/resources/pdf/sys/SCAS0085E_C10000... This Ebay listing quotes the line rate as 1khz, not sure where they got that: https://www.ebay.com/itm/4-Illumina-HiSeq-2000-Hamamatsu-S10405-Line-CCD-Camera-/372264830253 https://www.ebay.com/itm/4-Illumina-HiSeq-2000-Hamamatsu-S10... We were able to grab a live video feed from a single camera, that must have been 20fps at least, but we'll have to investigate fully. Yes, we are able adjust the gain and are able to focus both by moving the stage and by adjusting the more fine-grained piezo focus actuator (but we'd like to be able auto-focus using Micro Manager: https://micro-manager.org https://micro-manager.org).
- ak217 8y agoNice. I suspect there are quite a few Illumina engineers secretly cheering for you. I'm in touch with a community of microscopy folks which includes the maintainers of Micro Manager. I'll send this link around, I bet they'll be interested.