4 ms·

I am part of this project but just about to board a 2 hour flight. If you have any questions, feel free to post below and I will answer them later.

by kasbah 8y ago

ak217 8y ago
This is very cool. I hope you get some cool uses out of this. What's the max frame rate you can get out of the cameras (without tiling)? Are you able to control focus and camera gain?
- kasbah 8y ago
  Thanks, working out the max frame rate is a bit complicated by the fact it uses time delay integration (TDI) scanning. There are 4 TDI cameras each 2048 * 128 pixels. I couldn't find any official documentation (the cameras are Hamamatsu S10405 CCDs and there is one C10000-509 control board for 2 cameras each). I think it's the same sensor as this camera which quotes the line rate at 50khz. https://www.hamamatsu.com/resources/pdf/sys/SCAS0085E_C10000-801.pdf https://www.hamamatsu.com/resources/pdf/sys/SCAS0085E_C10000... This Ebay listing quotes the line rate as 1khz, not sure where they got that: https://www.ebay.com/itm/4-Illumina-HiSeq-2000-Hamamatsu-S10405-Line-CCD-Camera-/372264830253 https://www.ebay.com/itm/4-Illumina-HiSeq-2000-Hamamatsu-S10... We were able to grab a live video feed from a single camera, that must have been 20fps at least, but we'll have to investigate fully. Yes, we are able adjust the gain and are able to focus both by moving the stage and by adjusting the more fine-grained piezo focus actuator (but we'd like to be able auto-focus using Micro Manager: https://micro-manager.org https://micro-manager.org).
  - ak217 8y ago
    Nice. I suspect there are quite a few Illumina engineers secretly cheering for you. I'm in touch with a community of microscopy folks which includes the maintainers of Micro Manager. I'll send this link around, I bet they'll be interested.
GautamGoel 8y ago
I saw an ebay link for 4 cameras pulled from a HiSeq 2000. I am interested in using the cameras to build a digital microscope, but I'm not sure how easy it would be to interface the cameras to a PC, or if they need optics to work. Any ideas? Here is the link: https://www.ebay.com/itm/4-Illumina-HiSeq-2000-Hamamatsu-S10405-Line-CCD-Camera/372264830253?epid=19025744182&hash=item56acb6852d:g:eAcAAOSwQGZav8rc:rk:2:pf:0 https://www.ebay.com/itm/4-Illumina-HiSeq-2000-Hamamatsu-S10...

I am part of this project but just about to board a 2 hour flight. If you have any questions, feel free to post below and I will answer them later.

by kasbah 8y ago

I am part of this project but just about to board a 2 hour flight. If you have any questions, feel free to post below and I will answer them later.

ak217 8y ago

This is very cool. I hope you get some cool uses out of this. What's the max frame rate you can get out of the cameras (without tiling)? Are you able to control focus and camera gain?

kasbah 8y ago
Thanks, working out the max frame rate is a bit complicated by the fact it uses time delay integration (TDI) scanning. There are 4 TDI cameras each 2048 * 128 pixels. I couldn't find any official documentation (the cameras are Hamamatsu S10405 CCDs and there is one C10000-509 control board for 2 cameras each). I think it's the same sensor as this camera which quotes the line rate at 50khz. https://www.hamamatsu.com/resources/pdf/sys/SCAS0085E_C10000-801.pdf https://www.hamamatsu.com/resources/pdf/sys/SCAS0085E_C10000... This Ebay listing quotes the line rate as 1khz, not sure where they got that: https://www.ebay.com/itm/4-Illumina-HiSeq-2000-Hamamatsu-S10405-Line-CCD-Camera-/372264830253 https://www.ebay.com/itm/4-Illumina-HiSeq-2000-Hamamatsu-S10... We were able to grab a live video feed from a single camera, that must have been 20fps at least, but we'll have to investigate fully. Yes, we are able adjust the gain and are able to focus both by moving the stage and by adjusting the more fine-grained piezo focus actuator (but we'd like to be able auto-focus using Micro Manager: https://micro-manager.org https://micro-manager.org).
- ak217 8y ago
  Nice. I suspect there are quite a few Illumina engineers secretly cheering for you. I'm in touch with a community of microscopy folks which includes the maintainers of Micro Manager. I'll send this link around, I bet they'll be interested.

GautamGoel 8y ago

I saw an ebay link for 4 cameras pulled from a HiSeq 2000. I am interested in using the cameras to build a digital microscope, but I'm not sure how easy it would be to interface the cameras to a PC, or if they need optics to work. Any ideas? Here is the link: https://www.ebay.com/itm/4-Illumina-HiSeq-2000-Hamamatsu-S10405-Line-CCD-Camera/372264830253?epid=19025744182&hash=item56acb6852d:g:eAcAAOSwQGZav8rc:rk:2:pf:0 https://www.ebay.com/itm/4-Illumina-HiSeq-2000-Hamamatsu-S10...