3 ms·
Nebula uses 30X coverage for the free-with-sharing service. However, the $99 sequencing is about 0.4X.
by tonyoconnell 8y ago
Nebula uses 30X coverage for the free-with-sharing service. However, the $99 sequencing is about 0.4X.
- esyir 8y agoAnd that means everything. 0.4X coverage for whole genome is essentially worthless.
- tonyoconnell 8y agoCould you please explain why whole genome sequencing at 0.4x coverage is worthless? I would have thought it could provide a lot of value, for example, to understand inherited health risks and to predict drug response.
- zmmmmm 8y agowhere the coverage actually lands is completely random, so you couldn't count on learning anything about any specific part of the genome. But the parent is a bit wrong, it's not worthless. The reason people sequence at <1x is because you can still statistically derive a lot of useful info especially about larger variation - long segments of the genome that are deleted or duplicated etc can be inferred, and there is a technique called "imputation" which means if you measure one part of the genome well you can usually predict the nearby parts with surprising accuracy.
- fatboy93 8y agoBasically it means that you can derive single nucleotide variations to do genome wide association studies. However we do this low coverage of sequencing for plants to aid in the breeding programs
- akvadrako 8y agoWhere do you see this? I signed up for Nebula, but the 30X coverage is just greyed out.