Freezing isn't used by anybody. Cryonics organizations use vitrification, which preserves the brain (and body) in a sort of liquid-glass state. The antifreeze u

Freezing isn't used by anybody. Cryonics organizations use vitrification, which preserves the brain (and body) in a sort of liquid-glass state. The antifreeze used is massively toxic should the patient ever be warmed up, but at liquid-N2 temperatures it serves to preserve structure without the freezing damage you talk about, and nanotech-assisted recovery OR structural scanning would occur at that temperature. The newer techniques of room temperature preservation (with no hope of biological recovery, but very high hopes of structural preservation) apply structural scaffolding that keeps such neural microstructure in place, fossilizing the smallest relevant structures in a way a simple polish-and-scan destructive process can pick up, today. The latter point shows just how little jacquesm knows about this space. A rather large prize purse was recently won by a startup that preserved, through demonstrated scan-and-upload, a mammalian brain. Of course the brain-emulation software wasn't there to make a little VR mouse, but recovery of the brain's connectome was demonstrated.