I don't work in this area, but ECM scaffolds undergo significant change with age. Crosslinks and age-related remodelling degrade the mechanical properties of th

I don't work in this area, but ECM scaffolds undergo significant change with age. Crosslinks and age-related remodelling degrade the mechanical properties of the scaffold and interfere with normal cell migration and differentiation. You would want to start with a healthy organ before decellularising and reseeding.