3 ms·
It entirely depends why you’re modifying it. Introducing a transgene into E. coli can be done much easier than via CRISPR, true. But that’s not the only (or eve
by klmr 8y ago
It entirely depends why you’re modifying it. Introducing a transgene into E. coli can be done much easier than via CRISPR, true. But that’s not the only (or even main) use of CRISPR. I know of several relevant CRISPR experiments performed in E. coli that can’t really be performed any other way.
- dnautics 8y agoThose usually use a variant cas9 protein which is not what is included in Odin.
- klmr 8y agoNo, I'm talking about CRISPR/Cas9. For instance, there are experiments knocking out lots of loci simultaneously. I'm not aware of any other gene editing techniques that could be used by here, at least if you want to preserve some selectivity.
- dnautics 8y agoDon't know if they are more or less of a pain but lambda red I-SceI works quite well, and I think there is a kunkel based whole genome modifications option.