3 ms·
Near as I can tell the only real reference to CRISPR is that he purchased a plasmid that had been modified with CRISPR. Note: This is not what-so-ever like usin
by RosanaAnaDana 8y ago
Near as I can tell the only real reference to CRISPR is that he purchased a plasmid that had been modified with CRISPR. Note: This is not what-so-ever like using the CRISPR method to modify DNA. Modifying bacteria with a plasmid as he is demonstrating here is no where near its equivalent. Every introduction to biology course that I've ever seen performs the identical procedure (and generally with much higher rates of success).
- klmr 8y agoI think I got it: the plasmids (plural) contain the Cas9 and the gRNA targeting rpsL in the host organisms [1] (plus the DNA template for the homology directed repair). [1] http://www.the-odin.com/crispr-bacterial-guide/ http://www.the-odin.com/crispr-bacterial-guide/