4 ms·

$1000 for the Minion and 2 flow cells. $900 per flow cell after that[0]. For 30x coverage (standard) takes about 15 flow cells[1]. So looking at $12700 to DIY.

by jklehm 8y ago

$1000 for the Minion and 2 flow cells. $900 per flow cell after that[0]. For 30x coverage (standard) takes about 15 flow cells[1]. So looking at $12700 to DIY. [0] https://store.nanoporetech.com/flowcells.html https://store.nanoporetech.com/flowcells.html [1] https://nanoporetech.com/about-us/news/human-genome-minion https://nanoporetech.com/about-us/news/human-genome-minion

antpls 8y ago
I asked myself the same question some weeks ago, and I did a bit of basic research. I didn't keep the sources, but I found there are 2 types of sequencing : "draft" and "full". From what I found, prices are quickly dropping over the past years, and the "draft" sequencing is becoming more complete. Most likely, 23andMe does a "draft" sequencing. Also I also read the value of $1000 for a draft sequencing. I wonder, given there are about 3 millions deaths each year in USA, why isn't there some kind of government sponsored research to collect death people's genomes? Every year, there would be 3 millions more genomes in database. People already dead don't need to have data protection or insurances risk, and there would be a full health historic and cause of death available for each genomes.
- jghn 8y ago
  You’ll see 3 things in general: Genotyping: this is what 23andMe does. Here they’re looking at a few million locations on your genome to see what specific letter you have. These are the locations which are most typically different in humans Whole Exome Sequenckng (WES): full sequencing of your Exome. this is an oversimplification but the Exome has historically been viewed as where the “useful” DNA Whole Genome Sequencing (WGS): what the name implies. Can be quite useful in cancer and rare disease research, and were increasingly realizing how much of an oversimplification my explanation of WES is Almost all commercial “sequencing” that you see advertised is genotyping. And in the real sequencing world WES dominates WGS. In both cases it is due to cost. There are also a ton of options on all of these, such as how many SNPs are on the SNP chip in the genotyping case, or depth of coverage for WES/WGS
jghn 8y ago
It’s also extremely easy to introduce human error into this process unless you’ve had a lot of practice.

by jklehm 8y ago

antpls 8y ago

I asked myself the same question some weeks ago, and I did a bit of basic research. I didn't keep the sources, but I found there are 2 types of sequencing : "draft" and "full". From what I found, prices are quickly dropping over the past years, and the "draft" sequencing is becoming more complete. Most likely, 23andMe does a "draft" sequencing. Also I also read the value of $1000 for a draft sequencing. I wonder, given there are about 3 millions deaths each year in USA, why isn't there some kind of government sponsored research to collect death people's genomes? Every year, there would be 3 millions more genomes in database. People already dead don't need to have data protection or insurances risk, and there would be a full health historic and cause of death available for each genomes.

jghn 8y ago
You’ll see 3 things in general: Genotyping: this is what 23andMe does. Here they’re looking at a few million locations on your genome to see what specific letter you have. These are the locations which are most typically different in humans Whole Exome Sequenckng (WES): full sequencing of your Exome. this is an oversimplification but the Exome has historically been viewed as where the “useful” DNA Whole Genome Sequencing (WGS): what the name implies. Can be quite useful in cancer and rare disease research, and were increasingly realizing how much of an oversimplification my explanation of WES is Almost all commercial “sequencing” that you see advertised is genotyping. And in the real sequencing world WES dominates WGS. In both cases it is due to cost. There are also a ton of options on all of these, such as how many SNPs are on the SNP chip in the genotyping case, or depth of coverage for WES/WGS

jghn 8y ago

It’s also extremely easy to introduce human error into this process unless you’ve had a lot of practice.