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I believe there are services that will do whole genome seq for you for under $10k(and I assume won’t keep your data). You could then use Promethease for analysi

by breck 8y ago

I believe there are services that will do whole genome seq for you for under $10k(and I assume won’t keep your data). You could then use Promethease for analysis. I’m unaware of any way you could do self-DNA testing at home unless you wanted to spend $100k+ on equipment, reagents, etc. The Nanopore might be relevant but I haven’t looked into it much.

jklehm 8y ago
$1000 for the Minion and 2 flow cells. $900 per flow cell after that[0]. For 30x coverage (standard) takes about 15 flow cells[1]. So looking at $12700 to DIY. [0] https://store.nanoporetech.com/flowcells.html https://store.nanoporetech.com/flowcells.html [1] https://nanoporetech.com/about-us/news/human-genome-minion https://nanoporetech.com/about-us/news/human-genome-minion
- antpls 8y ago
  I asked myself the same question some weeks ago, and I did a bit of basic research. I didn't keep the sources, but I found there are 2 types of sequencing : "draft" and "full". From what I found, prices are quickly dropping over the past years, and the "draft" sequencing is becoming more complete. Most likely, 23andMe does a "draft" sequencing. Also I also read the value of $1000 for a draft sequencing. I wonder, given there are about 3 millions deaths each year in USA, why isn't there some kind of government sponsored research to collect death people's genomes? Every year, there would be 3 millions more genomes in database. People already dead don't need to have data protection or insurances risk, and there would be a full health historic and cause of death available for each genomes.
  - jghn 8y ago
    You’ll see 3 things in general: Genotyping: this is what 23andMe does. Here they’re looking at a few million locations on your genome to see what specific letter you have. These are the locations which are most typically different in humans Whole Exome Sequenckng (WES): full sequencing of your Exome. this is an oversimplification but the Exome has historically been viewed as where the “useful” DNA Whole Genome Sequencing (WGS): what the name implies. Can be quite useful in cancer and rare disease research, and were increasingly realizing how much of an oversimplification my explanation of WES is Almost all commercial “sequencing” that you see advertised is genotyping. And in the real sequencing world WES dominates WGS. In both cases it is due to cost. There are also a ton of options on all of these, such as how many SNPs are on the SNP chip in the genotyping case, or depth of coverage for WES/WGS
- jghn 8y ago
  It’s also extremely easy to introduce human error into this process unless you’ve had a lot of practice.
beaugunderson 8y ago
Full Genomes Corporation[1] will do WGS at 30x for $1295, WGS at 20x for $900, and Long Read WGS (newer technology) for $2900. For Prime Day, Dante Labs[2] had a sale on a 30x WGS for $349; it's still on sale for $499. Once you have a WGS you might consider donating it to the Harvard Personal Genomes Project[3], Open Humans[4], or putting it on GitHub[5]. [1]: https://www.fullgenomes.com/whole-genome-sequencing/ https://www.fullgenomes.com/whole-genome-sequencing/ [2]: https://us.dantelabs.com/products/whole-genome-sequencing-wgs-full-dna-analysis https://us.dantelabs.com/products/whole-genome-sequencing-wg... [3]: https://pgp.med.harvard.edu/ https://pgp.med.harvard.edu/ [4]: https://www.openhumans.org/ https://www.openhumans.org/ [5]: https://github.com/beaugunderson/genome https://github.com/beaugunderson/genome
- fapjacks 8y ago
  I've purchased a handful of Dante Labs 30x WGS kits on Amazon over the last couple of days. You didn't hear it from me, but the kits are still on sale on Amazon for $349... ... And an additional secret $100 discount during checkout. This is $250 WGS at 30x coverage. It should be front-page news.
  - tbrock 8y ago
    Can you provide a link or more info? I’m not seeing it. It seems to be at $499.
    - fapjacks 8y ago
      It looks like this listing ended about the same time that I posted about it, or at least in the time between my posting about it and six hours ago. Now there are only $499 kits by third-party sellers. But when there was still FBA inventory (fulfilled by Amazon), it was $349 plus the secret $100 "promotion" discount during checkout.
  - cncrnd 8y ago
    Are the results of WGS + analysis with promethease more insightful than the results of 23andMe?
crb002 8y ago
You could do it at Stanford or Iowa State for like $3k ? You would still have to prep the samples.

by breck 8y ago

jklehm 8y ago

$1000 for the Minion and 2 flow cells. $900 per flow cell after that[0]. For 30x coverage (standard) takes about 15 flow cells[1]. So looking at $12700 to DIY. [0] https://store.nanoporetech.com/flowcells.html https://store.nanoporetech.com/flowcells.html [1] https://nanoporetech.com/about-us/news/human-genome-minion https://nanoporetech.com/about-us/news/human-genome-minion

antpls 8y ago
I asked myself the same question some weeks ago, and I did a bit of basic research. I didn't keep the sources, but I found there are 2 types of sequencing : "draft" and "full". From what I found, prices are quickly dropping over the past years, and the "draft" sequencing is becoming more complete. Most likely, 23andMe does a "draft" sequencing. Also I also read the value of $1000 for a draft sequencing. I wonder, given there are about 3 millions deaths each year in USA, why isn't there some kind of government sponsored research to collect death people's genomes? Every year, there would be 3 millions more genomes in database. People already dead don't need to have data protection or insurances risk, and there would be a full health historic and cause of death available for each genomes.
- jghn 8y ago
  You’ll see 3 things in general: Genotyping: this is what 23andMe does. Here they’re looking at a few million locations on your genome to see what specific letter you have. These are the locations which are most typically different in humans Whole Exome Sequenckng (WES): full sequencing of your Exome. this is an oversimplification but the Exome has historically been viewed as where the “useful” DNA Whole Genome Sequencing (WGS): what the name implies. Can be quite useful in cancer and rare disease research, and were increasingly realizing how much of an oversimplification my explanation of WES is Almost all commercial “sequencing” that you see advertised is genotyping. And in the real sequencing world WES dominates WGS. In both cases it is due to cost. There are also a ton of options on all of these, such as how many SNPs are on the SNP chip in the genotyping case, or depth of coverage for WES/WGS
jghn 8y ago
It’s also extremely easy to introduce human error into this process unless you’ve had a lot of practice.

beaugunderson 8y ago

Full Genomes Corporation[1] will do WGS at 30x for $1295, WGS at 20x for $900, and Long Read WGS (newer technology) for $2900. For Prime Day, Dante Labs[2] had a sale on a 30x WGS for $349; it's still on sale for $499. Once you have a WGS you might consider donating it to the Harvard Personal Genomes Project[3], Open Humans[4], or putting it on GitHub[5]. [1]: https://www.fullgenomes.com/whole-genome-sequencing/ https://www.fullgenomes.com/whole-genome-sequencing/ [2]: https://us.dantelabs.com/products/whole-genome-sequencing-wgs-full-dna-analysis https://us.dantelabs.com/products/whole-genome-sequencing-wg... [3]: https://pgp.med.harvard.edu/ https://pgp.med.harvard.edu/ [4]: https://www.openhumans.org/ https://www.openhumans.org/ [5]: https://github.com/beaugunderson/genome https://github.com/beaugunderson/genome

fapjacks 8y ago
I've purchased a handful of Dante Labs 30x WGS kits on Amazon over the last couple of days. You didn't hear it from me, but the kits are still on sale on Amazon for $349... ... And an additional secret $100 discount during checkout. This is $250 WGS at 30x coverage. It should be front-page news.
- tbrock 8y ago
  Can you provide a link or more info? I’m not seeing it. It seems to be at $499.
  - fapjacks 8y ago
    It looks like this listing ended about the same time that I posted about it, or at least in the time between my posting about it and six hours ago. Now there are only $499 kits by third-party sellers. But when there was still FBA inventory (fulfilled by Amazon), it was $349 plus the secret $100 "promotion" discount during checkout.
- cncrnd 8y ago
  Are the results of WGS + analysis with promethease more insightful than the results of 23andMe?

crb002 8y ago

You could do it at Stanford or Iowa State for like $3k ? You would still have to prep the samples.