No, you can transfect the cells directly with Cas9 (the enzyme) and guide RNA, so you don't need to integrate the Cas9 gene into the genome, i.e. it won't be re

No, you can transfect the cells directly with Cas9 (the enzyme) and guide RNA, so you don't need to integrate the Cas9 gene into the genome, i.e. it won't be replicated and will vanish after cell division.