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It's efficient but not so precise and efficient that the modifications can't be seen as different that the background genome.
by inciampati 9y ago
It's efficient but not so precise and efficient that the modifications can't be seen as different that the background genome.
- gravelc 9y agoThat isn't really correct. If you delete a section of DNA, then the difference is that piece of DNA has been excised. Given that excision (or duplication) can occur naturally, there is no way of telling whether the change is due to CRISPR or not. It doesn't leave tell-tale signs. If there were a strict regulatory regime, there's simply no way of enforcing compliance. Of course it's simple when you put in something that's obviously exogenous - cheap genome sequencing will pick it up, hence why that will be regulated. Anything else, and you're relying on an honesty system.
- folli 9y agoThe precision will undoubtedly be increased. Remember that the system has been discovered approx. 10 years ago and the idea to use it for genome modifications (especially Cas9 from Streptococcus pyogenes) is only a couple of years old.