4 ms·
Resequencing of the modified genome is SOP, how else would you check that the right gene has been inserted/deleted in the right locus in the right way?
by folli 9y ago
Resequencing of the modified genome is SOP, how else would you check that the right gene has been inserted/deleted in the right locus in the right way?
- candiodari 9y agoSimple: let it grow. If it turns into purple zombies and eats the town (or otherwise shows unexpected gene expression), you failed. A variation on this is to also have it do an easy to check change, like various forms of fluorescence, then check for the easy to check ones and assume that if that one happened, the important one happened too.
- malnourish 9y agoNot all genes are expressed in the phenotype.
- candiodari 9y agoWell, true, but when you're changing genes, you're really looking to change the phenotype.