If you do ICSI with sperm taken directly from the testis, which is quite common, then the sperm never travel through the epididymis and therefore are not loaded

If you do ICSI with sperm taken directly from the testis, which is quite common, then the sperm never travel through the epididymis and therefore are not loaded with the microRNAs. So yes, you would certainly have removed the (is life good or shitty) signal that the sperm normally would carry with them. The Rando lab at UMass does a lot of this kind of research if you want to go down the rabbit hole...https://umassmed.edu/randolab/ https://umassmed.edu/randolab/