The computational barriers are definitely an issue. I thought I had read rumors on twitter that ONT was trying to implement read-until on one of their larger-sc

The computational barriers are definitely an issue. I thought I had read rumors on twitter that ONT was trying to implement read-until on one of their larger-scale sequencers (one of the ones with compute integrated). Barring that, the Cas9-mediated targeting method looks somewhat promising (https://www.youtube.com/watch?v=DGDH-FdoARM https://www.youtube.com/watch?v=DGDH-FdoARM). No need for amplification! Though for applications with human DNA you might run into some issues with the copy number of a sequence of interest if you’re starting out with a normal amount of genomic DNA.