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If you want to do a human or a dozen related humans, and then discard the equipment, is there anything cheaper? That is, without handing the DNA over to somebo
by burfog 9y ago
If you want to do a human or a dozen related humans, and then discard the equipment, is there anything cheaper?
That is, without handing the DNA over to somebody else.
- aroch 9y agoA MiniSeq or MiSeq would be cheaper by the time you do the 3 flow cell/person (minimum -- they claim 10-20GB/flow cell but that's bullshit -- so at least ~4K, probably closer to $8k of chemistry/person). You can get a used MiSeq and for ~$50k then its ~1500/person
- daemonk 9y agoIf you want to do absolutely everything yourself from DNA extraction to sequencing and data analysis, then yes, this is probably the cheapest way. But you also need to consider how you prepare your DNA to go into the Nanopore. It's a lot more investment than you might think. Extracting your DNA in a clean enough way for it to work with the Nanopore will require equipment/facilities that most people will not have access to (phenol/chloroform is the optimal way to extract DNA, which will require a fume hood and toxic chemical disposal). Depending on how you want to prep your DNA, you might need more specialized equipment/reagents (Ampure beads, ligation kits, end-prep/TA-tailing, etc).