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This is just one preliminary "teaser" paper by the group, not the scientific paper published later that got so much media attention. Here it is: http://journals
by Quanttek 9y ago
This is just one preliminary "teaser" paper by the group, not the scientific paper published later that got so much media attention. Here it is: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185809 http://journals.plos.org/plosone/article?id=10.1371/journal..... Also, measuring the mass of dead bugs is an acceptable method to measure insect population (you then either have a decline of the number or the weight) and the decline has been consistent and constant as well as conforming other long-time studies.
From their "methods and materials" section:
> Biomass data were collected and archived using a standardized protocol across 63 unique locations between 1989 and 2016 (resulting in 96 unique location-year combinations) by the Entomological Society Krefeld. The standardized protocol of collection has been originally designed with the idea of integrating quantitative aspects of insects in the status assessment of the protected areas, and to construct a long-term archive in order to preserve (identified and not-identified) specimens of local diversity for future studies. In the present study, we consider the total biomass of flying insects to assess the state of local entomofauna as a group.
> All trap locations were situated in protected areas, but with varying protection status: 37 locations are within Natura2000 sites, seven locations within designated Nature reserves, nine locations within Protected Landscape Areas (with funded conservation measures), six locations within Water Protection Zones, and four locations of protected habitat managed by Regional Associations. For all location permits have been obtained by the relevant authorities, as listed in the S1 Appendix. In our data, traps located in nutrient-poor heathlands, sandy grasslands, and dune habitats provide lower quantities of biomass as compared to nutrient nutrient-rich grasslands, margins and wastelands. As we were interested in whether the declines interact with local productivity, traps locations were pooled into 3 distinct habitat clusters, namely: nutrient-poor heathlands, sandy grassland, and dunes (habitat cluster 1, n = 19 locations, Fig 1A), nutrient-rich grasslands, margins and wasteland (habitat cluster 2, n = 41 locations, Fig 1B) and a third habitat cluster that included pioneer and shrub communities (n = 3 locations).
> Most locations (59%, n = 37) were sampled in only one year, 20 locations in two years, five locations in three years, and one in four years, yielding in total 96 unique location-year combinations of measurements of seasonal total flying insect biomass. Our data do not represent longitudinal records at single sites, suitable to derive location specific trends (e.g. [28]). Prolonged trapping across years is in the present context (protected areas) deemed undesirable, as the sampling process itself can negatively impact local insect stocks. However, the data do permit an analysis at a higher spatial level, i.e. by treating seasonal insect biomass profiles as random samples of the state of entomofauna in protected areas in western Germany.
> Malaise traps were deployed through the spring, summer and early autumn. They operated continuously (day and night), and catches were emptied at regular intervals, on average every 11.2 days (sd = 6.3). We collected in total 1503 trap samples, with an average of 16 (4–35) successive catches per location-year combination (Table 1). Between 1989 and 2016, a total of 53.54kg of invertebrates have been collected and stored, over a total trap exposure period of 16908 days, within an average of 176 exposure days per location-year combination. Malaise traps are known to collect a much wider diversity of insect species (e.g. [29–31]) as compared to suction traps (e.g. [28]) and are therefore considered superior as a method of collecting flying insects. On the basis of partial assessments, we can assume that the total number of insects included in 53.54 kg biomass represents millions of individuals.
> The sampling was standardized in terms of trap construction, size and design (identical parts), colors, type of netting and ground sealing, trap orientation in the field as well as slope at the trap location. Hence none of the traps differed in any of these field aspects. Our trap model was similar to the bi-colored malaise trap model by Henry Townes [32, 33]. The traps, collecting design, and accompanying methods of biomass measurement as designed and applied by the Entomological Society Krefeld are described elsewhere [34–36] and in S2 Appendix.
> Trap catches were stored in 80% ethanol solution, prior to weighing, and total insect biomass of each catch (bottle) was obtained based on a standardized measurement protocol by first subtracting fluid content. In order to optimally preserve samples for future species determination, the insects were weighed in an alcohol-wet state. First, the alcohol concentration in the vessels was stabilized to 80%, while this concentration was controlled with an areometer over a period of at least two days. In order to obtain biomass per sample with sufficient accuracy and comparability, the measuring process was fixed using a standardized protocol [34]. For this purpose the insects of a sample were poured onto a stainless steel sieve (10cm diameter) of 0.8 mm mesh width. This sieve is placed slightly obliquely (30 degrees) over a glass vessel. The skew position accelerates the first runoff of alcohol and thus the whole measuring procedure. The drop sequence is observed with a stopwatch. When the time between two drops has reached 10 seconds for the first time, the weighing process is performed with a laboratory scale. For the determination of the biomass, precision scales and analytical scales from Mettler company were used with an accuracy of at least 0.1g and controlled with calibrated test weights at the beginning of a new weighing series. In a series of 84 weightings of four different samples repeating this measurement procedure, an average deviation from the mean value of the measurement results of 0.4 percent was observed (unpublished results).