2 ms·
I'm very familiar with Sanger sequencing. Base for base it's one of the most accurate methods. It is by no means worthy of being considered a gold standard, it'
by AlexCoventry 9y ago
I'm very familiar with Sanger sequencing. Base for base it's one of the most accurate methods. It is by no means worthy of being considered a gold standard, it's just somewhat better. And the drawback is that dollar for dollar, it's pretty inefficient. You can genotype all the common variants using an Affymetrix gene chip,and base your inference on vastly more data. For forensic analysis, where the goal is to discriminate genomes, that would be much more accurate. Maybe go back and verify the most discriminatory/confirmatory loci with Sanger sequencing after that.