Practice as much as you can, in front of people whenever possible. All it takes is 3-4 smart people to come up with at least 80% of the questions you'll encount

Practice as much as you can, in front of people whenever possible. All it takes is 3-4 smart people to come up with at least 80% of the questions you'll encounter. Know those, and you'll be way ahead of most speakers. For the main talk, I found it also helps to write out a full transcript of my talk -- even though I usually don't follow it, it forces me to organize something coherently, and gives me something I can easily send to others for review. Best of luck, and enjoy Italy!