For synthesis, you don't need to know the exact pathway, but phosphorylation is very awkward synthetically, so you do need the enzyme for that (usually doesn't

For synthesis, you don't need to know the exact pathway, but phosphorylation is very awkward synthetically, so you do need the enzyme for that (usually doesn't have to be the exact one, though). Synthetic psilocin is already a thing, it's just not sold because it's illegal and rapidly oxidized by air. Understanding the biosynthesis of stuff is more of a gateway to messing with it where it is or transplanting it into bacteria (which can be cheaper than synthesis).