Thanks, I was looking for this info earlier (https://news.ycombinator.com/item?id=14448371 https://news.ycombinator.com/item?id=14448371): >"In our case, 56 zy

Thanks, I was looking for this info earlier (https://news.ycombinator.com/item?id=14448371 https://news.ycombinator.com/item?id=14448371): >"In our case, 56 zygotes were harvested from six pregnant females bred to six stud males and injected with CRISPRCas9." http://www.biorxiv.org/content/early/2017/06/23/154450 http://www.biorxiv.org/content/early/2017/06/23/154450 So they started with 56 zygotes, of which 11 survived injection of Crispr/etc. Of these 11, five and two mice showed some evidence of containing NHEJ/HDR cells, respectively. In the case of NHEJ, they do not tell us what percent of cells were "edited" per mouse (or I missed it when I read that paper earlier). However for HDR it was only about 1/3 and 1/5 for the two mice they found. So this suggests the "editing" is occurring not at the zygote stage, but somewhat later (based on the percentages, perhaps at the 4 or 8 cell stages). Assuming the edit/mouse rate is similar for NHEJ and HDR (somewhat dubious), it looks like 4x56 = 224 to 8x56 = 448 cells were treated with crispr/etc and ~ 7 got "edited". This gives ~1-3% "edits" per treated cell.