No, because DNA sequencing has lots of errors in it. This is why you might've seen numbers like '30x' or '50x coverage': to get a decent sequence, it gets done

No, because DNA sequencing has lots of errors in it. This is why you might've seen numbers like '30x' or '50x coverage': to get a decent sequence, it gets done again and again and hopefully the random errors wash out. Identical twins or clones are not perfectly genetically identical, but the differences are so rare that any difference in your sequence is almost certainly a sequencing error unless you do an expensive number of passes like 70x costing several times as much. Interestingly, this comes up a lot in criminal contexts now, since you can, if you want to spend the money, figure out which of two identical twins left DNA at a crime scene.