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From the abstract: Intravenously injected quadruplex sgRNAs/saCas9 AAV-DJ/8 excised HIV-1 proviral DNA and significantly reduced viral RNA expressi
by brut 9y ago
From the abstract:
Intravenously injected quadruplex sgRNAs/saCas9 AAV-DJ/8
excised HIV-1 proviral DNA and significantly reduced
viral RNA expression in several organs/tissues of Tg26
mice. In EcoHIV acutely infected mice, intravenously
injected quadruplex sgRNAs/saCas9 AAV-DJ/8 reduced
systemic EcoHIV infection, as determined by live
bioluminescence imaging.
Can a mod change the title to reflect the findings? The original title is "In Vivo Excision of HIV-1 Provirus by saCas9 and Multiplex Single-Guide RNAs in Animal Models" and should be kept. That way maybe some people wouldn't just reflex-upvote and actually at least read the abstract...
- JCzynski 9y agoThat abstract looks completely consistent with the submitted title. I don't see any reason to change it.
- alevskaya 9y ago"Eliminates" is a very loaded term in viral therapy. This paper doesn't show elimination of virus, it shows that the integrated viral genome can be excised in -some- cells by administration of adeno-associated virus harboring the CRISPR system.