I was a software developer at a company that produces LAL-based endotoxin tests. The best alternative[0] is an enzyme produced recombinantly in insect cells. It

I was a software developer at a company that produces LAL-based endotoxin tests. The best alternative[0] is an enzyme produced recombinantly in insect cells. It triggers an enzymatic reaction similar to LAL's (but more complicated). It is effective for testing most of the same products as LAL, and it has been accepted as an "alternative method" by the FDA and European Pharmacopeia. That means it can be used but with more extensive, expensive validation testing. There are some products it's not compatible with, and there is a small set of products that are more easily tested with the alternative enzyme. Also interesting that the article doesn't mention the standard before the LAL method was discovered: rabbits were injected with the test material and monitored for developing a fever. [0](http://www.lonza.com/products-services/pharma-biotech/endotoxin-detection/endotoxin-detection-assays/recombinant-factor-c-assay.aspx http://www.lonza.com/products-services/pharma-biotech/endoto...)