4 ms·

If you want to know why people are assuming NHEJ occurs at a double-stranded break, you might look at the dozen or so references in the first paragraph of https

• nonbel 10y ago
  Frequency of survival is shown in table 2... it ranges from 5/1,000 to 1/100,000. So cell death is a 200-100,000 times more common result than NHEJ using those methods. I have no idea how well that would correspond to the conditions we are interested here, but see no way this paper can be taken to support NHEJ is a more likely result than cell death. It also says: "Yeasts, like other eucaryotes, exhibit cell cycle arrest in response to DNA damage (17)."