3 ms·
Off the top of my head: Entirely new (smaller, thus more engineerable) Cas systems (1 month ago): http://www.nature.com/cr/journal/v27/n3/full/cr201721a.html h
by jfarlow 10y ago
Off the top of my head:
Entirely new (smaller, thus more engineerable) Cas systems (1 month ago):
http://www.nature.com/cr/journal/v27/n3/full/cr201721a.html http://www.nature.com/cr/journal/v27/n3/full/cr201721a.html
Variously engineered Cas9 proteins to reduce toxicity the over wild-type protein (two months ago):
https://www.ncbi.nlm.nih.gov/pubmed/28179977 https://www.ncbi.nlm.nih.gov/pubmed/28179977
Cas9-Inhibitors to help regulate activity (two months ago)
http://www.cell.com/cell/fulltext/S0092-8674(16)31683-X http://www.cell.com/cell/fulltext/S0092-8674(16)31683-X
And this doesn't include any ongoing research using other homing nuclease technologies like TALENs and Zn-Fingers that provide an entirely different set of tradeoffs but aren't as sexy, in part because they're harder to use.
- nonbel 10y agoI didn't see any data on improved survival/toxicity in those papers. The closest was Fig S4B/C of the last one (Rauch et al 2017), which shows some of the inhibitors are toxic too. So evidence for that may exist somewhere, but not there. Also, I think if they were serious about addressing this toxicity issue they would be include simple charts of the number of cells over time and number of "modified" cells over time. Then they (and the reader) can easily compare the toxicity and efficacy of different techniques.