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Influenza (and other (-)ssRNA viruses) are transcribed and replicated by an RNA-dependent RNA polymerase, that is, the protein makes +RNA out of a -RNA template
by virusduck 10y ago
Influenza (and other (-)ssRNA viruses) are transcribed and replicated by an RNA-dependent RNA polymerase, that is, the protein makes +RNA out of a -RNA template. This replication is happening in the cytoplasm, and for at least a limited amount of time, there is dsRNA present, although I am not sure of the on/off time for this. The paper you cite uses an antibody to detect dsRNA, which may not be an adequate marker for dsRNA ( the RNA nay be in an incompatible confirmation for the Ab, proximety to proteins may cause the RNA to be fixed to viral or other proteins during fixation procedure, etc).
- ihnorton 10y agoSon (2015) shows detection of dsRNA from (-)ssRNA viruses by immunofluorescence: http://jvi.asm.org/content/89/18/9383.full http://jvi.asm.org/content/89/18/9383.full They include this comment on the Weber (2006) paper cited above: > their lack of success might relate to the production of smaller amounts of dsRNA in negative-strand RNA virus infections, the use of the less sensitive J2 MAb, the choice of less than optimum times for p.i. sampling, or, possibly, technical issues.