4 ms·
as a big proponent of the cytof, i'm not sure that these guys used it in a novel capacity or even used it in its area of greatest strength... based off of what
by cryoshon 10y ago
as a big proponent of the cytof, i'm not sure that these guys used it in a novel capacity or even used it in its area of greatest strength... based off of what i can see, they did some simple immunophenotyping with less than a dozen markers, which traditionally be more suited for flow cytometry-- which they also used to detect GFP in a different section.
i like that the cytof is getting more popular, but people need to understand that the logistical commitment and jankiness of the system means that it should really only be used for its greatest strength: simultaneous detection of 30 variables on a small scale basis.
- apathy 10y agonah its biggest strength is to use as IMC for tissue sections ;-) but yes, reading more carefully, I suppose you're right. Then again, orthogonal validation is not a bad thing.