9 ms·
I once tried to grow magnetotactic bacteria. For the growth media, you had to prepare a witch's brew of unusual chemicals which sent me on a goose hunt around a
by bhickey 10y ago
I once tried to grow magnetotactic bacteria. For the growth media, you had to prepare a witch's brew of unusual chemicals which sent me on a goose hunt around a few friendly chem labs. The protocol called for making a couple of litres; smaller quantities would just exacerbate measurement errors. After cooking it up, you were supposed to add 2mL to the growth media and incubate the whole mess for four weeks.
Needless to say, I could never get them to grow.
Edit: I found the protocol!
NTA 1.5g
MgSO4 3.0g
MnSO4 0.5g
NaCl 1.5g
FeSO4 0.1g
CaCl2 0.1g
CoSO4 0.1g
ZnSO4 0.1g
CuSO4 0.025g
AlK(SO4)2 0.01g
H3BO3 0.01g
Na2MoO4 0.4g
NiCl2 0.01g
Phew. Did I mention: it was an anaerobe? Flushing an exotic growth media with nitrogen is a dull way to spend a Friday afternoon.