I mainly work with eukaryotes, so I don't know much microbio aside from generating vectors. Do we actually know enough about bacteria for this to work? I can se

I mainly work with eukaryotes, so I don't know much microbio aside from generating vectors. Do we actually know enough about bacteria for this to work? I can see it working for a small number of elements (anti-biotic resistance, promoter sites, cloned genes). But not for any behavior more complex than that?