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So it does prevent failure in NHEJ, doesn't it? But how does it prevent offtarget effects? I mean, if the wrong guide sequence is bound, an offtarget is mutated
by patall 11y ago
So it does prevent failure in NHEJ, doesn't it? But how does it prevent offtarget effects? I mean, if the wrong guide sequence is bound, an offtarget is mutated all along, isn't it?
- entee 11y agoI think the idea is to avoid NHEJ entirely. I would guess that it's going for base-excision-repair (BER), which is why it's currently targeting either C or G bases. If the wrong guide sequence is bound you're screwed no matter what, though I suspect they'd say that the error rate is low enough to be practically irrelevant. Not sure that's really true though, the genome is pretty big and if you screw up at even astonishingly low rates, you're still screwing up fairly frequently.
- jessriedel 11y agoDoes this answer your question, or are you asking something else? > The problem addressed by the new technique is not the usual one discussed with CRISPR, namely, off-target effects. That refers to changing a region of the genome other than the intended one. While early genome-editing experiments had that problem, there has been “tremendous progress” in fixing it, said Dr. Keith Joung of Massachusetts General Hospital.
- patall 11y agoThanks, thats what I was looking for. The first paragraph totally sounded like they were targetting offtargets, but in the end they dont.