4 ms·
Seems like it could be effective to use CRISPR/Cas9 to insert the HIV resistance gene mutation (CCR5-delta32, to replace the normal CCR5 gene) into bone marrow
by medymed 11y ago
Seems like it could be effective to use CRISPR/Cas9 to insert the HIV resistance gene mutation (CCR5-delta32, to replace the normal CCR5 gene) into bone marrow stem cells and then inject those into the person after testing the created cells to be HIV negative (or cutting the HIV out by means in this paper). If HIV negative bone marrow cells available were sufficient to do this it could be a 'cure' for the hematopoietic system. Or use HIV-negative induced pluripotent stem (IPS) cell with hematopoietic potential derived from a tissue not normally infected by HIV.
Such cells in principle would have a survival advantage over native cells and would effectively perform a bone marrow transplant, maybe helped along by some medicinal guidance. Pies in the sky, but using HIV-negative cells may avoid the problem of removing divergent sequences of HIV in different people and ineffective gDNAs.