4 ms·
Come on. Having actually done Westerns, there is absolutely zero chance that the "bp"=base pair in that figure is on purpose. Western blot is a protein blot and
by kyberias 11y ago
Come on. Having actually done Westerns, there is absolutely zero chance that the "bp"=base pair in that figure is on purpose. Western blot is a protein blot and they are always measured by their molecular weight (kDa). Base pairs would make no sense here. The numbers in the image are obviously kilo daltons.
How do we know the numbers are kilo daltons? Well, they say they're using β-tubulin as a loading control. β-tubulin's molecular weight is about 50kDa and that's what you can see in the image.
Also, look how the headers are not aligned properly in the image. As if the image is a draft.
- cybersiddhu 11y agoThe bp==base pair stands correct. It refers to Fig 2C, that shows the display of RT-PCR products(DNA made from RNA).