4 ms·
> For people who are involved in dealing with CRISPR/Cas9, what is the effective rate of gene editing done in vitro vs. in vivo? It will depend in part on the
by carbocation 11y ago
> For people who are involved in dealing with CRISPR/Cas9, what is the effective rate of gene editing done in vitro vs. in vivo?
It will depend in part on the tissue of interest and the delivery vector. In the mouse liver, the Broad group under Zhang has gotten efficiencies as high as 40% using Staphylococcal Cas9 [1].
1 = http://www.nature.com.sci-hub.io/nature/journal/v520/n7546/full/nature14299.html http://www.nature.com.sci-hub.io/nature/journal/v520/n7546/f...
- itcmcgrath 11y agoI found this + the op's comments fascinating purely because I wouldn't have been able to tell if both were real, or just fake technical talk of the ilk my friends and I sometimes do with meaningless software/hardware jargon as a joke, without Googling.
- noname123 11y agoWell, for the record, I am a programmer working at the Broad, a genomics center that is mired in the current CRISPR patent dispute (although I don't work with CRISPR at all). The answerer I believe is a MD/PhD at Mass General Hospital and before that a research scientist at the Broad working on genomics (and so is the author of the cited paper on mouse liver, small world here on HN). So I can be full of BS, but I don't think the answerer is. Tbh, I intentionally added a few more jargons more than necessary (I could have just asked "Can gene editing be effective in live humans T-cells vs. cells on a petri dish?") to field my question; but I intentionally did so to filter out the pop-sci, speculative futurists to get answers from people in life sciences research.