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Figure 1D is said to show "Detection of Cas9 protein by Western blot analysis", so what does "bp" stand for in that figure (above the ladder)? Usually it stands
by nonbel 11y ago
Figure 1D is said to show "Detection of Cas9 protein by Western blot analysis", so what does "bp" stand for in that figure (above the ladder)? Usually it stands for base pair but that doesn't make sense when looking at protein.
- kyberias 11y agoThat's an obvious error. The scale in the figure is kilo-daltons (for proteins). Bp scale is used in figure 1C (RNA). Perhaps they copy pasted that image when fabric... I mean polishing the images.
- leemailll 11y agoNot knowing hacker news and pubpeer overlaps
- cryoshon 11y agoBase pairs. I don't know the exact math and haven't ever done Westerns, but there's almost certainly a way of converting protein size (typically expressed in kDa) to base pairs, since theoretically a large enough quantity of bp would weigh as much as a protein of a given size. The trick here is to understand that Western blots use gel electrophoresis to detect protein size, meaning that heavier proteins will not traverse as far as lighter proteins.
- kyberias 11y agoCome on. Having actually done Westerns, there is absolutely zero chance that the "bp"=base pair in that figure is on purpose. Western blot is a protein blot and they are always measured by their molecular weight (kDa). Base pairs would make no sense here. The numbers in the image are obviously kilo daltons. How do we know the numbers are kilo daltons? Well, they say they're using β-tubulin as a loading control. β-tubulin's molecular weight is about 50kDa and that's what you can see in the image. Also, look how the headers are not aligned properly in the image. As if the image is a draft.
- cybersiddhu 11y agoThe bp==base pair stands correct. It refers to Fig 2C, that shows the display of RT-PCR products(DNA made from RNA).