0.05 was first proposed by Fisher himself, which is 2 sigma if you look at a normal distribution (95% falls within 2 standard deviations[1]). Indeed, physics (

0.05 was first proposed by Fisher himself, which is 2 sigma if you look at a normal distribution (95% falls within 2 standard deviations[1]). Indeed, physics (generally particle physics, such as the Higgs Boson) goes for 5 or 6 sigma. In biology, 0.05 is used because, well, it's hard otherwise. The cost, time, effort required for plants, animals, and even cells get exorbitant quickly, and are subject to many, many variations (animals generally have additional IACUC limits on procedures and numbers). I could make arguments about how particles obey laws while biology is at least a few levels abstracted over that fundamental behavior, but xkcd has already done that. I personally feel that p<0.05 is absurd, and the entire biological field NEEDS to move to p<0.01. There's a movement toward that end, and it's about damn time.[3] 1: https://en.wikipedia.org/wiki/68%E2%80%9395%E2%80%9399.7_rule https://en.wikipedia.org/wiki/68%E2%80%9395%E2%80%9399.7_rul... 2: https://xkcd.com/435/ https://xkcd.com/435/ & https://xkcd.com/1052/ https://xkcd.com/1052/ & https://xkcd.com/1520/ https://xkcd.com/1520/ spring to mind 3: A lot of internet ink has been spilled on the topic, but http://www.nature.com/nmeth/journal/v12/n3/full/nmeth.3288.html http://www.nature.com/nmeth/journal/v12/n3/full/nmeth.3288.h... & http://www.pnas.org/content/110/48/19313.long http://www.pnas.org/content/110/48/19313.long & http://www.nature.com/news/scientific-method-statistical-errors-1.14700 http://www.nature.com/news/scientific-method-statistical-err... are good places to start.