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I think a lot of the mutations can be attributed to infidelities of single cell sequecing. The amplification of DNA that has to be done has a tendency to incorp
by Mieroels 11y ago
I think a lot of the mutations can be attributed to infidelities of single cell sequecing. The amplification of DNA that has to be done has a tendency to incorparate errors. If you start with a single copy of DNA then an copy error early in amplification will look like a mutation.
The neuron data is compared to non-single cell sequencing of heart cells.
- daemonk 11y agoThey actually did something kinda clever. They were able to calculate/model a false positive rate by looking at the homozygosity of the X chromosome. Since the samples were male, there should only be one X, so any heterozygosity would have to be errors. Not saying this is an absolute correct way of going about this (no one really knows), but it shows they did at least thought about this issue.
- thundara 11y agoIf you look in the second paragraph, they say they used multiple-displacement amplification (MDA). This method reduces the amount of times that any copied DNA is re-amplified, allowing them to significantly reduce the issue you bring up. [1] https://en.wikipedia.org/wiki/Multiple_displacement_amplification https://en.wikipedia.org/wiki/Multiple_displacement_amplific...