5 ms·
Maybe. Maybe not. Viral therapies suffer from one problem: viruses. (They might be infectious, or not. They might be attacked by the immune system or not.) CRI
by mudil 11y ago
Maybe. Maybe not. Viral therapies suffer from one problem: viruses. (They might be infectious, or not. They might be attacked by the immune system or not.) CRISPR technique does not need an external vector.
- timrpeterson 11y agoHow does the cas9 protein get in?
- kbenzle 11y agoBoth protein and guide RNA need to be expressed in the organism, so viral, bacterial or mechanical introduction of DNA is still necessary.
- gww 11y agoCIRSPR requires a method to deliver the nuclease complexes to cellular targets in a living organism. I believe the only methods tried so far used adenoviruses.
- pcrh 11y agoA few recent papers have shown that it can be done by directly introducing the required proteins.